The role of phospholipides in the uptake of amino acids by Ehrlich ascites carcinoma cells.

The phospholipides of Ehrlich ascites carcinoma of the mouse are capable of rapidly incorporating amino acids following in vivo or in vitro incubation of the cells with the labeled compounds. Glucose (0.005 M) stimulated the in vitro uptake of leucine and alanine by the phospholipides of the ascites cells. The amino acids appeared to be readily liberated by the phospholipides into the amino acid pool or protein constituents of the cell but could not be separated from the purified phospholipides electrophoretically. The presence of the amino acids could be demonstrated by paper chromatography following acid hydrolysis of the phospholipide complex.